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You are using a standard microscope with a 0.10NA 4x objective and switch to a 0.65NA 40x objective. What are the acceptance angles for each? Compare and comment on the values. Which would you use first to locate the target area on of your specimen?
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Final Answer
0.10NA lens: $11^\circ$
0.65NA lens: $81^\circ$
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OpenStax College Physics Solution, Chapter 26, Problem 31 (Problems & Exercises) (2:27)

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